In situ hybridization of zebra fish with pax 2.1

More pictures from the lab, this time we made in situ preparations of different stades of zebra fish embryos. This means that you basically make the mRNA (in this case for the morphogen pax 2.1) visible. Pax 2.1 is expressed (as you can see HERE) in the midbrain hindbrain boundary (early stade), in the otic placode and pronephric mesoderm (later) and after 24 hours in the spinal chord neurons, the otic vesicle, the anterior retina, the otic stalk and the pronephric duct.

Pax 2.1 is a transcription factor with a homeobox domain

Express yourself!
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by Charles Betz
06/30/06. 07:13:40 pm. 93 words, 2871 views. Categories: Uni Aktuell ,

Mini Apéro Thanks to the Affolter Lab

Today the Affolter Lab generously offered us a small reward for our work this week, seems like we've done a good job...See the pics HERE

So that's why the Biozentrum needs that much ice!
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by Charles Betz
06/30/06. 06:03:46 pm. 30 words, 1015 views. Categories: Uni Aktuell ,

Morphogenetic movements

β-Catenin, Bozozok, Chordin, Dickkopf, Squint, Cyclops, One-eyed pinhead, No tail, Brachyury and Floating head...Yeah, floating head that's what I got right now, that's why I decided to go over it again and draw a scheme by myself...For all of the non-developmental biology specialists among us, these are names of Zebra fish genes or more precisely names of their gene products. The fundamental question is how can you make a bilateral (head, tail, dorsal and ventral axis) fish (or vertebrate for that matter) from a symmetrical sphere...The solution is a very complex and not entirely understood interaction network between morphogenetic gradients that can either stimulate or inhibit other protein or mRNA gradients and so on...Basically, the first breach in the symmetry is the entry of the sperm into the egg.

If this sounds like Chinese, please consider the possibility to not try to understand it, most people (except biology students of course) can happily live without ever knowing what a morphogenetic gradient is...

Hope I got it right, please send in you feedback so that I can correct it when necessary!

Drämmli Map Of Basel? My Secret Plan To Conquer The World??
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by Charles Betz
06/29/06. 07:48:10 pm. 189 words, 2271 views. Categories: Labonews , 2 comments »

GFP zebra fish under the fluorescence microscope

Added the latest pics, here's an appetizer for today's menu...Smells like fish!
Is it a zebra? Is it a fish? Find out!
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Find more HERE

by Charles Betz
06/29/06. 05:35:41 pm. 21 words, 1172 views. Categories: Uni Aktuell ,

Luxembourg - Basel (via Trier, Koblenz, Mainz, Frankfurt, Nürnberg and Karlsruhe, of course)

As Marc mentioned in his post earlier, our trip to the world cup 2006 Netherlands vs. Portugal was a full success. So as I do not want to reserve you the pictures I took, along with my impressions, I'll write one article too:

Full story »

by Charles Betz
06/28/06. 06:06:30 pm. 739 words, 1296 views. Categories: News , 2 comments »

Marc screams: Forca!

On Sunday Charles and I went to Nürnberg to see Portugal vs Holland. Charles arrived in Frankfurt late in the morning and we got into a rather adventerous train journey a few hours later.

First the locomotive had to be changed due to technical difficulties... Then we had to stop right in the middle, the whole train smelled like burned plastic, and the personel was running around like crazy. But of course, it was just a precaution.

With about 40 minutes delay we arrived in Nürnberg, but never got the free drinks that were promised.

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by marc
06/28/06. 05:36:56 pm. 204 words, 2907 views. Categories: Hello World ,

In Other News : Dechorionated Zebra Fish Embryos

Danio rerio is an important model organism for studying vertebrate development. During this and the next days, our pratical work with Prof. Affolter's lab will focus on these fascinating organisms. We analyze different developmental stages of these embryos and thereby maybe get some insight into our own fate...Right now, we are doing some in situ hybridization experiments, stay tuned on Friday to see what 3 days of lab work can produce...

All hail Captain Iglo
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by Charles Betz
06/28/06. 03:39:21 pm. 76 words, 699 views. Categories: Labonews, Uni Aktuell ,

The Left Handed DNA Hall Of Fame


A funny site featuring all publications in the recents years where the DNA molecule is shown to left-handed (although we all know that most of this earth's DNA is right-handed of course). Some mentions include covers of big journals, or commemorative coins etc...Check it out now!

by Charles Betz
06/28/06. 08:35:10 am. 47 words, 881 views. Categories: Labonews ,

Photography rules


MIKE JOHNSTON writes in this interesting article about so-called established rules in photography...Very interesting post!

by Charles Betz
06/28/06. 07:50:45 am. 16 words, 1640 views. Categories: Photography ,

Take a bow for the Royals

Most of you might not be too familiar with our Grand Duke Henri de Luxembourg, so I thought you might be interested in hearing why we Luxembourgers are so proud of our Heng and his family. Or are we? Find it out yourself!

The wild bunch
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by Charles Betz
06/27/06. 06:42:05 pm. 537 words, 4597 views. Categories: Gossip , 2 comments »

Mothers against Dpp

Red=Phalloidin + GFP Clones by mitotic FRT Recombination
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Check out more of our Drosophila Melanogaster slides we prepared today here. The red channel is the phalloidin staining, the green ones are obtained via the FRT/MARCM system with a GFP gene. Basically, the green cells are the ones that underwent mitotic recombination.

by Charles Betz
06/27/06. 06:14:36 pm. 49 words, 424 views. Categories: Photography ,

D'Lëtzebuerg brennt

Last Friday was a massive fire in Clausen (Luxembourg) in a depot of the Mousel brewary...I took the pic from my garden...
Burning in Clausen
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Fire in the hole
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by Charles Betz
06/27/06. 08:03:04 am. 33 words, 540 views. Categories: News ,

It's raining cats and dogs!

Finally, Flo, our new Leonberger puppy arrived at its new home...She's such a sweet dog, I will put some more pictures to the gallery...Anyhow, I think that pictures say more than a thousand words, so why waste my time:

Flo, our new dog
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More to be found HERE

by Charles Betz
06/26/06. 08:11:10 pm. 51 words, 380 views. Categories: News , 2 comments »

Ons Hémécht - Faakelzuch - Nationalfeierdag

So as you probably heard, the last weekend was kind of hard for me because there was just simply so much to do and so little time...

First of all, on Thursday, I skipped some lectures to get the early train that takes me to Luxembourg in about 3.5 hours, the reason being that this was the evening before national day, which means lots of people streaming into our capital.

Line and her sister Jil picked me up at the central station and we went back to my place to meet Flo finally...This will feature in another article...So, I packed my things, took my uniform and went to the HOGA where we meet every year with my scout group to grill before going to the Faakelzuch or torch procession, if one can name it like that. I did manage to convince Annik and Lena to watch over me while I checked the quality of this year's strawberries harvest...Still pretty green in comparison with the ones Line gave me from her own garden...

Anouk, a cub (Wëllefchen) from our scout group
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by Charles Betz
06/26/06. 07:47:19 pm. 326 words, 1830 views. Categories: News ,

Nora says

hello all! probeiern dat hei lo mol:D

by norres
06/26/06. 04:47:57 pm. 8 words, 1063 views. Categories: Hello World ,

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